CRISPR-Cas系統(tǒng)新的應(yīng)用：SHERLOCK檢測技術(shù)

華裔科學(xué)家張鋒利用CRISPR-Cas新系統(tǒng)，研發(fā)出了新的快速，便宜，高靈敏度的診斷工具，可以用于檢測諸如寨卡病毒感染和登革熱感染等的疾病，這一進(jìn)展也有助于便捷檢測和診斷許多其它的病原體，該結(jié)果發(fā)表在著名雜志科學(xué)（Science）上。不同于Cas9，新的Cas蛋白是Cas13a，具有在剪切掉目標(biāo)RNA后還能保持活性，可以“插手”剪切其他非目標(biāo)RNA的特點(diǎn)。運(yùn)用Cas13a的“連帶剪切”特性，再添加一種特定RNA熒光標(biāo)記物，并使用特殊擴(kuò)增技術(shù)，依靠體溫?cái)U(kuò)增檢測樣本中的RNA，就形成了一個(gè)準(zhǔn)確檢測到單個(gè)核酸分子的檢測系統(tǒng)。

該系統(tǒng)命名為“神探夏洛克（SHERLOCK，Specific High Sensitivity Enzymatic Reporter UnLOCKing）”。可以用于檢測血清、尿液和唾液中低濃度的寨卡病毒，而且可以用于檢測病毒載量，區(qū)分不同的菌株，甚至能分辨獨(dú)有不同耐藥突變的肺炎菌株和不同的癌癥突變。

又是一篇科學(xué)（Science）文章，祝賀華裔大神在“神”的道路上繼續(xù)高速前進(jìn)。 

原文摘要：

Nucleic acid detection with CRISPR-Cas13a/C2c2

Abstract：Rapid, inexpensive, and sensitive nucleic acid detection may aid point-of-care pathogen detection, genotyping, and disease monitoring. The RNA-guided, RNA-targeting CRISPR effector Cas13a (previously known as C2c2) exhibits a "collateral effect" of promiscuous RNAse activity upon target recognition. We combine the collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this Cas13a-based molecular detection platform, termed SHERLOCK (Specific High Sensitivity Enzymatic Reporter UnLOCKing), to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify cell-free tumor DNA mutations. Furthermore, SHERLOCK reaction reagents can be lyophilized for cold-chain independence and long-term storage, and readily reconstituted on paper for field applications.